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Carol A. LoopstraAssociate ProfessorDepartment of Forest Science
Ph.D. Forestry and Genetics (1992)
Molecular Biology of Forest Trees The primary areas of interest in our laboratory are understanding wood development and drought resistance in pines. Most of the work focuses on analysis of gene expression including microarrays, real-time RT-PCR, and promoter cloning and analysis. We have recently become very interested in looking at genetic differences in gene expression within and between populations. Unlike most crop plants, loblolly pine still has much of the genetic variation that was present before domestication. Materials in tree improvement programs can usually be traced to the county of origin. Therefore, we can try to relate differences in gene expression to traits of commercial importance. We have identified differences in gene expression between populations for genes associated with drought resistance and cell wall composition. Using real-time RT-PCR, we are looking at members of multigene families and have found that different members of an AGP (arabinogalactan-protein) family, the cellulose synthase family, and a dehydrin are regulated very differently. The xylem work was supported by the National Science Foundation. The drought stress work is supported by the Texas Higher Education Coordinating Board Advanced Technology Program.
GENE / MEPS 411 Biotechnology for Crop Improvement (credit hours: 3) FRSC 203 Dendrology (credit hours: 3)
Yang S-H, van Zyl L, No E-G, and Loopstra CA (2004) Microarray analysis of genes preferentially expressed in differentiating xylem of loblolly pine (Pinus taeda). Plant Science 166(5): 1185-1195 Zhang Y, Brown G, Whetten R, Loopstra CA, Neale D, and Sederoff RR. (2003) An arabinogalactan-protein associated with secondary cell wall formation in differentiating xylem of loblolly pine. Plant Molecular Biology- 52:91-102 No E-G and Loopstra CA (2000) Hormonal and developmental regulation of two loblolly pine xylem arabinogalactan-proteins. Physiologia Plantarum – 110: 524-529. No E-G, Zhou Y, and Loopstra CA. (2000) Sequences upstream and downstream of two xylem-specific pine genes influence their expression. Plant Science 160: 77-86. Loopstra CA, Puryear JD, and No E-G. (2000) Purification and cloning of an arabinogalactan-protein from xylem of loblolly pine. Planta –210: 686-689 |
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