Primers should be at least 18mer and desalted. Each sample will require 10 picomoles primer per reaction. Calculate the melting temperature of your primer. One of several methods is:
Tm = [(A+T) x 2°C] + [(G+C) x 4°C]
This will determine the cycle sequencing program used to incorporate the fluorescent dideoxynucleotides.
10 pmole/rxp sequencing primer is used.